Logo Helmholtz Zentrum München

Project description

To achieve accurate packaging of DNA into chromatin, cells need to precisely control the concentration of histone proteins, the building blocks of nucleosomes. Typically, histone production is tightly linked to DNA replication during S-phase, to ensure that histones are produced when needed. However, at this point we do not know when this cell-cycle-dependence of histone production is established in the early mammalian embryo, nor how embryos are able to produce the right amount of histones despite exponentially increasing DNA concentration during the early stages of development.

We will use molecular biology and imaging approaches to obtain a quantitative understanding of histone biogenesis during early mouse development. Manipulation of the embryo will then be used to disentangle the mechanistic contribution of DNA content, developmental stage, and cell size.

Related literature:

K.M. Schmoller and J.M. Skotheim (2015), The Biosynthetic Basis of Cell Size Control. Trends in Cell Biology, 25, 793-802

This interdisciplinary project will be a close collaboration with the group of Prof. Dr. Maria Elena Torres-Padilla (Epigenetics and cell-fate in early mammalian development) and requires the candidate to perform experiments in both labs.

 

 

We use cookies to improve your experience on our Website. We need cookies to continuously improve the services, to enable certain features and when embedding services or content of third parties, such as video player. By using our website, you agree to the use of cookies. We use different types of cookies. You can personalize your cookie settings here:

Only cookies permitted that are required for the basic functionality of our websites.
Permit cookies that help us to analyse the page views and user behavior on our websites. We only use this information for improving our services.
Permit embedded content and cookies from third party providers. This setting enables the full use of our web services (e.g. displaying videos).
Please find more information in our privacy statement.

There you may also change your settings later.